A-1851A: A Rapid Method for Ribosome Subunit Isolation: Part 2--Using the High-Capacity Swinging Bucket MLS-50 Rotor in an OptimaTM MAX Tabletop Ultracentrifuge

Introduction The separation performance of the OptimaTM MAX ultracentrifuge is demonstrated by isolating a bacterial ribosome using two high-capacity rotors. This work is described in two separate application information bulletins: • Part 1 (document number A-1850A) describes conditions for pelleting an intact 70 S ribosome using differential centrifugation with a fixed angle MLA-80 rotor and an 8-mL tube. • Part 2 (this document) describes conditions for preparing and separating 30 S and 50 S ribosomal subunits using rate zonal density gradient centrifugation with a swinging bucket MLS-50 rotor and a 5-mL tube.